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| Names | |
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| Other names Seminaphtharhodafluorescein; Seminaphthorhodafluor | |
| Identifiers | |
3D model (JSmol) | |
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PubChem CID | |
CompTox Dashboard (EPA) | |
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| Properties | |
Chemical formula | C24H15NO4 |
| Molar mass | 381.387 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
Seminaphtharhodafluor or SNARF is a fluorescent dye that changes color with pH. It can be used to construct optical biosensors that use enzymes that change pH.
The absorption peak of the derivative carboxy-SNARF at pH 6.0 is at wavelength (515 and) 550 nm, while that at pH 9.0 is at 575 nm.
The emission peak of carboxy-SNARF at pH 6.0 is at wavelength 585 nm, while that at pH 9.0 is at 640 nm.[1]
SNARF-1 can serve as a substrate for the MRP1 (multidrug resistance-associated protein-1) drug transporter, to measure the activity of the MRP1 transporter. For this purpose, an acetomethoxyester group is added to SNARF-1. Cellular esterases cleave off SNARF-1, and its transport out of the cells can be measured by following the loss of fluorescence from the cells.[2]
References
- ^ "5-(and-6)-Carboxy SNARF™-1". www.thermofisher.com.
- ^ Weekes, M. P.; Tan, S. Y. L.; Poole, E.; Talbot, S.; Antrobus, R.; Smith, D. L.; Montag, C.; Gygi, S. P.; Sinclair, J. H.; Lehner, P. J. (12 April 2013). "Latency-Associated Degradation of the MRP1 Drug Transporter During Latent Human Cytomegalovirus Infection". Science. 340 (6129): 199–202. Bibcode:2013Sci...340..199W. doi:10.1126/science.1235047. PMC 3683642. PMID 23580527.