| Quorum regulatory RNA | |
|---|---|
) | |
| Identifiers | |
| Symbol | Qrr |
| Alt. Symbols | Qrr1–5 |
| Rfam | RF00378 |
| Other data | |
| RNA type | sRNA |
| Domain(s) | Vibrio |
| PDB structures | PDBe |
Qrr (Quorum regulatory RNA)[1] is a non-coding RNA that is thought to be involved in the regulation of quorum sensing in Vibrio species. It is believed that these RNAs, guided by a protein, Hfq, can mediate the destabilisation of the quorum-sensing master regulators LuxR/HapR/VanT mRNAs.[2][3]
Qrr operates as part of a negative feedback loop which regulates the shift in cell state from that of low density populations to that in high density populations.[4] In Vibrio harveyi, Qrr binds and represses expression of the luxR mRNA transcript, a master regulator of quorum-sensing genes.[5] At higher population densities, increased autoinducer concentration leads to termination of qrr transcription hence derepression of luxR.[4]
There are 5 different qrr genes (Qrr1–5) in V. harveyi; of these, qrr2, 3 and 4 are activated by LuxR.[6]
References
- ^ Lenz, DH; Mok KC; Lilley BN; Kulkarni RV; Wingreen NS; Bassler BL (2004). "The Small RNA Chaperone Hfq and Multiple Small RNAs Control Quorum Sensing in Vibrio harveyi and Vibrio cholerae". Cell. 118 (1): 69–82. doi:10.1016/j.cell.2004.06.009. PMID 15242645.
- ^ Svenningsen SL, Tu KC, Bassler BL (2009). "Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing". EMBO J. 28 (4): 429–439. doi:10.1038/emboj.2008.300. PMC 2632942. PMID 19165149.
- ^ Tu, K.; Waters, C.; Svenningsen, S.; Bassler, B. (Nov 2008). "A small-RNA-mediated negative feedback loop controls quorum-sensing dynamics in Vibrio harveyi". Molecular Microbiology. 70 (4): 896–907. doi:10.1111/j.1365-2958.2008.06452.x. ISSN 0950-382X. PMC 2680268. PMID 18808382.
- ^ a b Tu KC, Long T, Svenningsen SL, Wingreen NS, Bassler BL (2010). "Negative Feedback Loops Involving Small Regulatory RNAs Precisely Control the Vibrio harveyi Quorum-Sensing Response". Mol Cell. 37 (4): 567–579. doi:10.1016/j.molcel.2010.01.022. PMC 2844700. PMID 20188674.
- ^ Miyamoto, CM; Smith, EE; Swartzman, E; Cao, JG; Graham, AF; Meighen, EA (October 1994). "Proximal and distal sites bind LuxR independently and activate expression of the Vibrio harveyi lux operon". Molecular Microbiology. 14 (2): 255–262. doi:10.1111/j.1365-2958.1994.tb01286.x. PMID 7830570.
- ^ Tu, KC; Waters, CM; Svenningsen, SL; Bassler, BL (November 2008). "A small-RNA-mediated negative feedback loop controls quorum-sensing dynamics in Vibrio harveyi". Molecular Microbiology. 70 (4): 896–907. doi:10.1111/j.1365-2958.2008.06452.x. PMC 2680268. PMID 18808382.
Further reading
- Shao, Y; Bassler, BL (Apr 3, 2014). "Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae". Molecular Microbiology. 92 (5): 921–930. doi:10.1111/mmi.12599. PMC 4038675. PMID 24698180.