Amphinase

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Structures	Swiss-model
Domains	InterPro

Amphinase
	Crystallographic structure of amphinase.
Identifiers
Organism	Rana pipiens
Symbol	n/a
PDB	2P7S
UniProt	P85073
Other data
EC number	3.1.27
Search for
Structures	Swiss-model
Domains	InterPro

Amphinase is a ribonuclease enzyme found in the oocytes of the Northern leopard frog (Rana pipiens). Amphinase is a member of the pancreatic ribonuclease protein superfamily and degrades long RNA substrates.^[2] Along with ranpirnase, another leopard frog ribonuclease, amphinase has been studied as a potential cancer therapy due to its unusual mechanism of cytotoxicity tested against tumor cells.^[3]

References

^ Singh UP, Ardelt W, Saxena SK, Holloway DE, Vidunas E, Lee HS, Saxena A, Shogen K, Acharya KR (August 2007). "Enzymatic and structural characterisation of amphinase, a novel cytotoxic ribonuclease from Rana pipiens oocytes". Journal of Molecular Biology. 371 (1): 93–111. doi:10.1016/j.jmb.2007.04.071. PMID 17560606.
^ Singh UP, Ardelt W, Saxena SK, Holloway DE, Vidunas E, Lee HS, Saxena A, Shogen K, Acharya KR (August 2007). "Enzymatic and structural characterisation of amphinase, a novel cytotoxic ribonuclease from Rana pipiens oocytes". Journal of Molecular Biology. 371 (1): 93–111. doi:10.1016/j.jmb.2007.04.071. PMID 17560606.
^ Ardelt W, Shogen K, Darzynkiewicz Z (June 2008). "Onconase and amphinase, the antitumor ribonucleases from Rana pipiens oocytes". Current Pharmaceutical Biotechnology. 9 (3): 215–25. doi:10.2174/138920108784567245. PMC 2586917. PMID 18673287.

[1] Singh UP, Ardelt W, Saxena SK, Holloway DE, Vidunas E, Lee HS, Saxena A, Shogen K, Acharya KR (August 2007). "Enzymatic and structural characterisation of amphinase, a novel cytotoxic ribonuclease from Rana pipiens oocytes". Journal of Molecular Biology. 371 (1): 93–111. doi:10.1016/j.jmb.2007.04.071. PMID 17560606.

[2] Singh UP, Ardelt W, Saxena SK, Holloway DE, Vidunas E, Lee HS, Saxena A, Shogen K, Acharya KR (August 2007). "Enzymatic and structural characterisation of amphinase, a novel cytotoxic ribonuclease from Rana pipiens oocytes". Journal of Molecular Biology. 371 (1): 93–111. doi:10.1016/j.jmb.2007.04.071. PMID 17560606.

[3] Ardelt W, Shogen K, Darzynkiewicz Z (June 2008). "Onconase and amphinase, the antitumor ribonucleases from Rana pipiens oocytes". Current Pharmaceutical Biotechnology. 9 (3): 215–25. doi:10.2174/138920108784567245. PMC 2586917. PMID 18673287.

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