HART may refer to:
- Halt All Racist Tours, New Zealand
- Heterogeneous Aerial Reconnaissance Team
- Highway Addressable Remote Transducer Protocol
- Hillsboro Artists' Regional Theatre
- Human Assisted Reproductive Technology
- Hypervelocity Aircraft Rocket, Tactical
Transportation
- Hillsborough Area Regional Transit, Florida
- Honolulu Authority for Rapid Transportation, Hawaii
- Housatonic Area Regional Transit, Connecticut
- Huntington Area Rapid Transit, Long Island, New York
Response or rescue teams
- Hazardous Area Response Team, UK
- High Acuity Response Team, a type of Rapid response team (medicine)
Science and technology
- Highly Active Anti-Retroviral Therapy
- HART Communications Protocol (Highway Addressable Remote Transducer) - a communication protocol used in the process industries.