AFB may refer to:
- Armed Forces Bikers, a UK-based motorcycle charity to assist former members of the armed forces
- Acid-fast bacilli
- Air Force Base
- Air Force Brat (children of Air Force personnel)
- American Farm Bureau
- American flatbow, a style of bow used in archery
- American foulbrood, a honeybee disease
- American Foundation for the Blind
- Spoken Gulf Arabic (SIL code)
- AFB, the forward-backward asymmetry
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